Product Description

CDC5L Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90276-50, BS90276-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99459(Human) Q6A068(Mouse) O08837(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC

All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: Cdc5L (Cell division cycle 5-like protein, Pombe Cdc5-related protein) is a DNA-binding protein encoded by the human gene CDC5L. Cdc5L contains 2 HTH myb-type DNA-binding domains and may shuttle between cytoplasm and nucleus. It is involved in cell cycle control and may act as a transcription activator. CDC5L is a spliceosomal protein that is highly conserved across species. It is a member of a protein group that comprise the core of spliceosomal complexes and are essential for pre-mRNA splicing. Cdc5L is involved in the second catalytic step of pre-mRNA splicing, which involves cleavage at the 3' splice site and the ligation of the exons. This process releases the intact intron lariat. A chromosomal aberration involving Cdc5L is found in multicystic renal dysplasia. This aberration is caused by a translocation with USF-2.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CDC5L polyclonal antibody detects endogenous levels of CDC5L protein.

Bioworld Molecular Weight: 92/100 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CDC5L on different lysates using anti-CDC5L antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat, ICC staining CDC5L in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Cdc5-like protein, CDC5L, CDC5, CDC5 cell division cycle 5-like (S. pombe), Cdc5 related protein, CDC5-LIKE, Cdc5-like protein, Cdc5l, CDC5L, CEF1, Cell division cycle 5 S. pombe homolog of, Cell division cycle 5-like protein, dJ319D22.1, KIAA0432, PCDC5RP, Pombe Cdc5 related protein, Pombe Cdc5-related protein,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified