Product Description

Cdc6 (phospho-S54) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS4036-50, BS4036-100

Swiss-Prot: Q99741

Host: Rabbit

Cdc6 (phospho-S54) Polyclonal Antibody-BS4036

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Cdc6 (S54) polyclonal antibody detects endogenous levels of Cdc6 only when phosphorylated at Ser54.

Bioworld Molecular Weight: ~ 62 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of p-Cdc6 (S54) polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate treated with serum starvation
Lane 2: Raw264.7 cell lysate treated with serum starvation
Lane 3: H9C2 cell lysate treated with serum starvation, Immunohistochemistry (IHC) analysis of p-Cdc6 (S54) pAb in paraffin-embedded human lung carcinoma tissue.

Alternative Name: Cell division control protein 6 homolog; CDC6-related protein; Cdc18-related protein; HsCdc18; p62(cdc6); HsCDC6; CDC6; CDC18L; p62 Cdc6

Immunogen: Synthetic phosphopeptide derived from human Cdc6 around the phosphorylation site of Serine 54.

Modification: Phosphorylation

Conjugate: Unconjugated