CDk1 Polyclonal Antibody-BS90278

CDk1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90278-50, BS90278-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06493

Host: Rabbit

Reactivity: Human, Mouse, rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500
ICC:1:200
IHC:1:200
FC:1:100-1:200

Background: Cdk1 is a small protein (approximately 34 kilodaltons), and is highly conserved. Cdk1 is comprised mostly by the bare protein kinase motif, which other protein kinases share. Cdk1, like other kinases, contains a cleft in which ATP fits. When bound to its cyclin partners, Cdk1 phosphorylation leads to cell cycle progression. Given its essential role in cell cycle progression, Cdk1 is highly regulated. Most obviously, Cdk1 is regulated by its binding with its cyclin partners. Cyclin binding alters access to the active site of Cdk1, allowing for Cdk1 activity; furthermore, cyclins impart specificity to Cdk1 activity. At least some cyclins contain a hydrophobic patch which may directly interact with substrates, conferring target specificity. Furthermore, cyclins can target Cdk1 to particular subcellular locations.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CDk1 polyclonal antibody detects endogenous levels of CDk1 protein.

Bioworld Molecular Weight: 34 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CDk1 on different cell lysates using anti-CDk1 antibody at 1/500 dilution.
Positive control:
Lane 1: MCF-7
Lane 2: Jurkat
Lane 3: PC12
Lane 4: HepG2
Lane 5: Hela
Lane 6: NIH/3T3
Lane 7: Mouse liver
Lane 8: SKBR3, ICC staining CDk1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CDK1, Cdc 2, Cdc2, CDC28A, CDK 1, CDK1, CDK1, CDKN1, CELL CYCLE CONTROLLER CDC2, Cell division control protein 2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, Cell division protein kinase 1, Cell Divsion Cycle 2 Protein, Cyclin Dependent Kinase 1, Cyclin-dependent kinase 1, DKFZp686L20222, MGC111195, p34 Cdk1, p34 protein kinase, P34CDC2,

Immunogen: peptide

Conjugate: Unconjugated

Modification: Unmodified