Product Description

CDK4 Monoclonal Antibody

Product: Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: MB66868-50, MB66868-100

Sizes: 50µl, 100µl

Swiss-Prot: P11802

Host: Mouse

Reactivity: Human, Mouse

Applications: WB, IHC

Background: The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control . Biological regulation by 14-3-3 is mediated through phosphorylation-dependent protein-protein interactions . Two different phospho-Ser-containing motifs are found within nearly all known 14-3-3 binding proteins . Motif is found in critical regulatory proteins including Bad, cdc25C, FKHRL1, PKC and c-Raf . Phospho-14-3-3 Binding Motif Polyclonal and Monoclonal Antibodies provide powerful tools for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery.

Product: Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of CDK4 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CDK4 expression in A431 (A), Hela (B), K562 (C), MCF7 (D), NIH3T3 (E) whole cell lysates., Immunohistochemical analysis of CDK4 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 30 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3

Immunogen: Recombinant fusion protein of human CDK4. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified