Cdk4 Polyclonal Antibody-BS90282

Cdk4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90282-50, BS90282-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11802(Human) P30285(Mouse) P35426(Rat)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:5,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression . Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin upregulates Cdk4 activity, whereas binding to the Cdk inhibitor p16 downregulates Cdk4 activity. Activation of the Cdk4-cyclin complexes requires phosphorylation on a single threonyl residue of Cdk4, catalyzed by a Cdk-activating protein (CAK).

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Cdk4 polyclonal antibody detects endogenous levels of Cdk4 protein.

Bioworld Molecular Weight: 34 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cdk4 on Hela cells lysates using anti-Cdk4 antibody at 1/1,000 dilution., ICC staining Cdk4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CDK4, Cdk 4, cdk4, CDK4 protein, CDK4, Cell division kinase 4, Cell division protein kinase 4, CMM 3, CMM3, Crk3, Cyclin dependent kinase 4, Cyclin-dependent kinase 4, Melanoma cutaneous malignant 3, MGC14458, p34 cdk4, PSK J3, PSK-J3,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified