CDK5RAP3 Monoclonal Antibody-MB10463

CDK5RAP3 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10463-50, MB10463-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q96JB5

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-P, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Background: Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation (PubMed:12054757, PubMed:12737517). Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation (PubMed:17785205, PubMed:20228063). Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint (PubMed:15790566, PubMed:19223857). Through its interaction with CDKN2A/ARF and MDM2 may induce MDM2-dependent p53/TP53 ubiquitination, stabilization and activation in the nucleus, thereby promoting G1 cell cycle arrest and inhibition of cell proliferation (PubMed:16173922). May play a role in the unfolded protein response, mediating the ufmylation of multiple proteins in response to endoplasmic reticulum stress (PubMed:23152784). May also play a role in the rupture of the nuclear envelope during apoptosis (PubMed:23478299). May regulate MAPK14 activity by regulating its dephosphorylation by PPM1D/WIP1 (PubMed:21283629).

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 57 kDa; Observed MW: 70 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CDK5RAP3 in K562, Hela lysates using CDK5RAP3 antibody., Immunohistochemistry analysis of paraffin-embedded Human breast cancer using CDK5RAP3 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Alternative Name: C53; IC53; LZAP; HSF-27; MST016; PP1553; OK/SW-cl.114

Immunogen: A synthetic peptide of human CDK5RAP3

Conjugate: Unconjugated

Modification: Unmodified