Product Description

Cdk6 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90284-50, BS90284-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q00534(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk6 is known to associate with cyclins D1, D2 and D3 and to be involved with the G1/S transition of the cell cycle. Multiple inhibitors of Cdk6 have been identified, including p18 and p19. These inhibitors bind to both free and complexed Cdk6, and they inhibit the activity of the cyclin D-bound Cdk6.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Cdk6 polyclonal antibody detects endogenous levels of Cdk6 protein.

Bioworld Molecular Weight: 37 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cdk6 on different lysates using anti-Cdk6 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: K562
Lane 3: Hela, ICC staining Cdk6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CDK6, CDK 6, CDK6, CDK6, Cell division protein kinase 6, Crk 2, Crk2, Cyclin dependent kinase 6, Cyclin-dependent kinase 6, MGC59692, p40, PLSTIRE, Serine/threonine protein kinase PLSTIRE, Serine/threonine-protein kinase PLSTIRE, STQTL11,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified