Product Description

CDK9 (Phospho-T186) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64533-50, BS64533-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P50750

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB:1:500~1:1000

Background: A family of proteins designated cyclin dependent kinases (Cdks) are critical regulators of cell cycle progression. Cdk family members, including Cdc2 p34, Cdk1–9, PISSLRE, KKIALRE, PITSLRE and PCTAIRE 1–3 are constitutively expressed throughout the cell cycle. Cdc2 p34 activity peaks during mitosis and Cdk2 activity rises in late G1 or early S phase. Cdk4 and Cdk6 are critically involved in G1 to S phase progression. The functions of Cdk3, Cdk5b, PISSLRE, KKIALRE and PCTAIRE 1–3 are less well defined. Cdk9 (also designated PITALRE) has been shown to specifically phosphorylate the retinoblastoma protein. The more recently cloned Drosophila protein, P-TEFb, is thought to be the homolog of mammalian PITALRE. P-TEFb has been shown to be required for HIV Tat transcriptional activation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CDK9 (Phospho-T186) polyclonal antibody detects endogenous levels of CDK9 protein only when phosphorylated at Thr186.

Bioworld Molecular Weight: ~ 42 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CDK9 (Phospho-T186) polyclonal antibody at 1:500 dilution
Lane A: PC3 whole cell lysate
Lane B: The Liver tissue lysate of Mouse
Lane C: The Liver tissue lysate of Rat

Alternative Name: Cyclin-dependent kinase 9; C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit; CDC2L4; TAK

Immunogen: Synthetic phosphopeptide derived from human CDK9 around the phosphorylation site of Threonine 186.

Conjugate: Unconjugated

Modification: Phosphorylation