Product Description

Cdk9 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90287-50, BS90287-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P50750(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, IP,

All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: A family of proteins designated cyclin dependent kinases (Cdks) are critical regulators of cell cycle progression. Cdk family members, including Cdc2 p34, Cdk1–9, PISSLRE, KKIALRE, PITSLRE, and PCTAIRE 1–3 are constitutively expressed throughout the cell cycle. Cdc2 p34 activity peaks during mitosis and Cdk2 activity rises in late G1 or early S phase. Cdk4 and Cdk6 are critically involved in G1 to S phase progression. The functions of Cdk3, Cdk5b, PISSLRE, KKIALRE and PCTAIRE 1–3 are less well defined. Cdk9 (also designated PITALRE) has been shown to specifically phosphorylate the retinoblastoma protein. The more recently cloned Drosophila protein, P-TEFb, is thought to be the homolog of mammalian PITALRE. P-TEFb has been shown to be required for HIV Tat transcriptional activation.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Cdk9 polyclonal antibody detects endogenous levels of Cdk9 protein.

Bioworld Molecular Weight: 43/37 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cdk9 on different lysates using anti-Cdk9 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Jurkat
Lane 3: A431, ICC staining Cdk9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CDK9, C-2K, CDC2 related kinase, CDC2L4, Cdk 9, Cdk9, CDK9, Cell division cycle 2-like protein kinase 4, Cell division protein kinase 9, CTK1, Cyclin dependent kinase 9, Cyclin-dependent kinase 9, PITALRE, Serine/threonine-protein kinase PITALRE, TAK, Tat associated kinase complex catalytic subunit,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified