Product Description

CDKN1A/p21CIP1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80290-50, BS80290-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P38936

Host: Rabbit

Reactivity: Human

Applications: WB, IHC, IP,

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:100 | IP,1:50 - 1:200

Background: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of MCF7 cells, using CDKN1A/P21cip1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s., Immunohistochemistry of paraffin-embedded human liver using CDKN1A/p21CIP1 Rabbit pAb at dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug CDKN1A/p21CIP1 antibody. Western blot was performed from the immunoprecipitate using CDKN1A/p21CIP1 antibody at a dilution of 1:500., Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug CDKN1A/p21CIP1 antibody. Western blot was performed from the immunoprecipitate using CDKN1A/p21CIP1 antibody at a dilution of 1:500.

Alternative Name: CDKN1A; CAP20; CDKN1; CIP1; MDA-6; P21; SDI1; WAF1; p21CIP1; cyclin-dependent kinase inhibitor 1

Immunogen: Recombinant fusion protein of human CDKN1A/p21CIP1(NP_000380.1).

Conjugate: Unconjugated

Modification: Unmodified