CDKN3 Polyclonal Antibody-BS6562

CDKN3 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS6562-50, BS6562-100

Swiss-Prot: Q16667

Host: Rabbit

CDKN3 Polyclonal Antibody-BS6562

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene belongs to the dual specificity protein phosphatase family. It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. This gene was reported to be deleted, mutated, or overexpressed in several kinds of cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 24KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of MCF7, using CDKN3 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s., Immunofluorescence analysis of MCF7 cells using CDKN3 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: CDKN3; CDI1; CIP2; KAP; KAP1

Immunogen: Recombinant fusion protein of human CDKN3(NP_005183.2).

Modification: Unmodified

Conjugate: Unconjugated