CDX2 Monoclonal Antibody-MB66116

CDX2 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66116-50, MB66116-100

Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Swiss-Prot: Q99626

Host: Mouse

Reactivity: Human

Applications: IHC

All Applications: IHC (1/100 - 1/300)

Background: CDX2, a homeobox domain-containing transcription factor, is a master regulator of the trophectoderm, the layer that gives rise to extra-embryonic tissues in mammalian development . CDX2 is also involved in intestinal development, and gain of expression or loss of expression has been associated with various human malignancies, such as Barrett's esophagus and colorectal cancer . Mouse embryonic stem cells deficient in CDX2 display limited hematopoietic progenitor development and altered Hox gene expression , pointing to a role for CDX2 in Hox gene regulation. CDX2 is also implicated in the aberrant expression of Hox genes in human AML cell lines .

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemical analysis of CDX2 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6. 0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 33 kDa

Specificity: Recognizes endogenous levels of CDX2 protein.

Alternative Name: CDX3; Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human CDX2. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified