Product Description

CEBPZ Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76805-50, BS76805-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q03701

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene belongs to the CEBP family. The encoded protein plays a role in cellular response to environmental stimuli through a transcriptional process that involves heat shock factors, conserved DNA elements (heat shock elements or HSEs) and CCAAT boxes. The protein acts as a DNA-binding transcriptional activator and regulates the heat-shock protein 70 (HSP70) promoter in a CCAAT-dependent manner. The protein is also involved in cell growth and differentiation, particularly, hematopoietic differentiation. Methylation of the promoter of this gene or mutations within the gene may be correlated with occurance of acute myeloid leukemia (AML).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of various cell lines, using CEBPZ antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 140kDa

Alternative Name: CEBPZ; CBF; CBF2; HSP-CBF; NOC1

Immunogen: Recombinant fusion protein of human CEBPZ(NP_005751.2).

Conjugate: Unconjugated

Modification: Unmodified