Product Description

Cellubrevin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90293-50, BS90293-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15836(Human) P63024(Mouse) P63025(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC, IF, IHC, FC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Vesicle-associated membrane proteins, known as VAMPs, also designated synaptobrevins, include VAMP-1, VAMP-2, VAMP-3 (cellubrevin), and synaptotagmin, a protein that may function as an inhibitor of exocytosis. VAMP proteins are vesicular factors that are important components of the machinery controlling docking and/or fusion of secretory vesicles with their target membrane. Synaptosomal-associated proteins, known as SNAPs, including alpha- and gamma-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and syntaxin. Pancreatic beta-cells express VAMP-2 and VAMP-3, and either one or both of these proteins selectively control Ca2+-mediated insulin secretion. In addition, VAMP-2 and VAMP-3 are expressed on GLUT4-containing vesicle membranes isolated from 3T3-Ll adipocytes and are important components of the insulin-dependent translocation of GLUT4 to the cell surface in adipocytes.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Cellubrevin polyclonal antibody detects endogenous levels of Cellubrevin protein.

Bioworld Molecular Weight: 11 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cellubrevin on A549 (1), 293T(2) cell lysates using anti-Cellubrevin antibody at 1/1,000 dilution., ICC staining Cellubrevin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: VAMP-3, CEB, VAMP3, SYB3, CEB, Cellubrevin, Synaptobrevin 3, Synaptobrevin-3, VAMP 3, VAMP-3, VAMP3, VAMP3, Vesicle associated membrane protein 3, Vesicle-associated membrane protein 3,

Immunogen: Synthetic peptide conjugated to KLH within N-terminal human Cellubrevin.

Conjugate: Unconjugated

Modification: Unmodified