Product Description

CENPA (Phospho-S7) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS67429-50, BS67429-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Modμlation of chromatin structure plays a critical role in the regμlation of transcription and replication of the eukaryotic genome. The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regμlating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modμlate chromatin structure. CENP-A, also known as the chromatin-associated protein CSE4 (capping-enzyme suppressor 4-p), is an essential histone H3 variant that replaces canonical histone H3 in centromeric heterochromatin. The greatest divergence between CENP-A and canonical histone H3 occurs in the amino-terminal tail of the protein, which binds linker DNA between nucleosomes and facilitates proper folding of centromeric heterochromatin . The amino-terminal tail of CENP-A is also required for recruitment of other centromeric proteins (CENP-C, hSMC1, hZW10), proper kinetochore assembly and chromosome segregation during mitosis . Additional sequence divergence in the histone fold domain is responsible for correct targeting of CENP-A to the centromere . Many of the functions of CENP-A are regμlated by phosphorylation . Aurora A-dependent phosphorylation of CENP-A on Ser7 during prophase is required for proper targeting of Aurora B to the inner centromere in prometaphase, proper kinetochore/microtubμle attachment and proper alignment of chromosomes during mitosis.

Category: Primary Antibody

Reactivity: Human, Rat

Host: Rabbit

Applications: WB, IF/ICC

Alternate Names: Histone H3-like centromeric protein A, Centromere autoantigen A, Centromere protein A, CENP-A

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CENPA with a site at pS7. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/200)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of CENPA with a site at pS7 protein.

Molecular Weight: ~ 43 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P49450

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only