Product Description

CENPC Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90294-50, BS90294-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q03188(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC IF, IHC FC IP,

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:200
IP:1:10-1:50

Background: A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The evolutionarily conserved Centromere Protein-C, CENP-C, is a kinetochore assembly protein. CENP-C is located on the fibers of the kinetochore and constitutes a kinetochore organizing center that tightly associates with DNA. CENP-C is necessary for the formation of a functional centromere, which indicates that CENP-C is important for mitotic progression. In addition, CENP-C is lost from centromeres during herpes simplex virus 1 infection, causing substantial structural changes in the kinetochore, which suggests that the structure of CENP-C is regulated during the cell cycle.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: CENPC polyclonal antibody detects endogenous levels of CENPC protein.

Bioworld Molecular Weight: 107 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CENPC on K562 cell lysate using anti-CENPC antibody at 1/1,000 dilution., ICC staining CENPC in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: CENP-C, CENP-C 1, CENP-C 1, CENP-C, CENPC, CENPC1, Centromere autoantigen C, Centromere autoantigen C1, Centromere protein C 1, Centromere protein C, hcp-4, ICEN7, Interphase centromere complex protein 7, MIF2,

Immunogen: Recombinant protein within human CENPC aa 400-600.

Conjugate: Unconjugated

Modification: Unmodified