Product Description

CEP290 (F812) Polyclonal Antibody

Catalogue Numbers: BS3831-50, BS3831-100

Sizes: 50µl, 100µl

Alternative Name: Centrosomal protein of 290 kDa; Cep290; Bardet-Biedl syndrome 14 protein; Cancer/testis antigen 87; CT87; Nephrocystin-6; Tumor antigen se2-2; CEP290; BBS14; KIAA0373; NPHP6

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O15078

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: CEP290 activates ATF4 mediated transcription and is required for the correct localization of ciliary and phototransduction proteins in retinal photoreceptor cells; may play a role in ciliary transport processes. CEP290 is ubiquitously expressed; strongly in placenta and weakly in brain. There are two named isoforms. Defects in CEP290 are a cause of Joubert syndrome type 5 (JBTS5), Senior-Loken syndrome type 6 (SLSN6), Leber congenital amaurosis type 10 (LCA10) and Meckel syndrome type 4 (MKS4). Antibodies against CEP290 are present in sera from patients with cutaneous T cell lymphomas, but not in the healthy control population.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CEP290 (F812) polyclonal antibody detects endogenous levels of CEP290 protein.

Bioworld Molecular Weight: ~ 290 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CEP290 (F812) polyclonal antibody at 1:500 dilution
Lane 1: HCT116 whole cell lysate(40ug)
Lane 2: SGC7901 whole cell lysate(40ug)
Lane 3: COS-7 whole cell lysate(40ug)
Lane 4: A549 whole cell lysate(40ug)
Lane 5: CT26 whole cell lysate(40ug)
Lane 6: AML-12 whole cell lysate(40ug)
Lane 7: PC12 whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 781-830 of Human CEP290.

Conjugate: Unconjugated

Modification: Unmodified