Cerebellin 4 (K178) Polyclonal Antibody-BS3282

Cerebellin 4 (K178) Polyclonal Antibody

Catalogue Numbers: BS3282-50, BS3282-100

Sizes: 50µl, 100µl

Alternative Name: Cerebellin-4; Cerebellin-like glycoprotein 1; CBLN4; CBLNL1; UNQ718/PRO1382; Cerebellin4

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NTU7

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: Cerebellin (CER), which was originally isolated from rat cerebellum, is a hexadecapeptide derived from a larger precursor called Cerebellin 1, also designated precerebellin 1 or Cbln1. Four propeptides, Cerebellin 1, Cerebellin 2 (Cbln2), Cerebellin 3 (Cbln3) and Cerebellin 4 (Cbln4), comprise the precerebellin subfamily within the C1q protein family. Cerebellin family members act as transneuronal regulators of synapse development and synaptic plasticity in various brain regions. Cerebellin and its metabolite, des-Ser(1)Cer, are also expressed in several extra-cerebellar tissues, including adrenal gland. Cerebellin 1, 2 and 3 assemble into homomeric and heteromeric complexes, thereby influencing each other’s degradation and secretion. Cerebellin 3 is not able to form homomeric complexes, and can only be secreted upon forming a heteromeric complex with Cerebellin 1. Decreased concentrations of Cerebellin have been found in the brain of patients with olivopontocerebellar atrophy (OPCA) and Shy-Drager syndrome, suggesting a role for Cerebellin in the pathology of these diseases.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Cerebellin 4 (K178) polyclonal antibody detects endogenous levels of Cerebellin 4 protein.

Bioworld Molecular Weight: ~ 27 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Cerebellin 4 (K178) pAb at 1:500 dilution
Lane 1: The Brain tissue lysate of Mouse(40ug)
Lane 2: The Brain tissue lysate of Rat(40ug)
Lane 3: U-87MG whole cell lysate(20ug)
Lane 4: L02 whole cell lysate(20ug)

Immunogen: Synthetic peptide, corresponding to amino acids 148-197 of Human Cerebellin 4.

Conjugate: Unconjugated

Modification: Unmodified