CERKL Polyclonal Antibody-BS67058

CERKL Polyclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: BS67058-50, BS67058-100

Sizes: 50µl, 100µl

Swiss-Prot: Q49MI3

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

Background: Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions.

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of CERKL protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CERKL expression in HCT116 (A), SGC7901 (B), A549 (C), HEK293T (D) whole cell lysates., Immunohistochemical analysis of CERKL staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 62 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Ceramide kinase-like protein

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CERKL. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified