Product Description

CHAC1 Polyclonal Antibody

Catalogue Numbers: BS60131-50, BS60131-100

Sizes: 50µl, 100µl

Alternative Name: Cation transport regulator-like protein 1; Blocks Notch protein; Botch; BOTCH

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BUX1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: CHAC1 (ChaC, cation transport regulator homolog 1 (E. coli)) is a 264 amino acid cytoplasmic protein that belongs to the ChaC family. Induced by chemical activators of the unfolded protein response (UPR) pathway, including DTT, tunicamycin and thapsigargin, CHAC1 is a pro-apoptotic member of this pathway and may mediate the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Overexpression of CHAC1 may lead to an increase in apoptosis, while suppression of CHAC1 results in subdued apoptosis. Existing as two alternatively spliced isoforms, the gene encoding CHAC1 maps to human chromosome 15q15.1. Encoding more than 700 genes, chromosome 15 is made up of approximately 106 million base pairs and comprises about 3% of the human genome. Angelman, Prader-Willi and Marfan syndromes are associated with loss of function or deletion of genes that are encoded by chromosome 15.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CHAC1 polyclonal antibody detects endogenous levels of CHAC1 protein.

Bioworld Molecular Weight: ~ 24-28 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CHAC1 polyclonal antibody at 1:500 dilution
Lane 1: Hela whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human CHAC1.

Conjugate: Unconjugated

Modification: Unmodified