Product Description

CHAMP1 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-64089-60, E-AB-64089-120, E-AB-64089-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: CHAMP1

Target Synonym: CHAMP1; C13orf8; CAMP; CHAMP; MRD40; ZNF828

Research Areas: Epigenetics and Nuclear Signaling

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse

Application: IF

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q96JM3

Background: CHAMP1 (chromosome alignment maintaining phosphoprotein 1), also known as ZNF828 or C13orf8, is a 812 amino acid protein containing one C2H2-type zinc finger. CHAMP1 is required for the proper alignment of chromosomes during metaphase, undergoing CDK1-dependent phosphorylation at multiple sites during mitosis. The phosphorylation counteracts the negative chromosomal alignment regulation of the zinc-finger domain of CHAMP1. One region of CHAMP1, the FPE region, is responsible for spindle and kinetochore localization, which is essential for proper chromosome alignment. CHAMP1 interacts with MAD2L2, PGOZ, CBX1, CBX3 and CBX5, and may recruit CENPE and CENPF to the kinetochore. The CHAMP1 gene is located on chromosome 13 and is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat and chicken.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human CHAMP1 (NP_115812.1).

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: IF 1:50-1:100

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only