ChAT Polyclonal Antibody-BS65860

ChAT Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65860-50, BS65860-100

Swiss-Prot: P28329

Host: Rabbit

ChAT Polyclonal Antibody-BS65860

Storage and Stability: Store at 4°C short term. Aliquot and store at -33°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010].

Purification and Purity: affinity purified by Protein A

Specificity: ChAT Polyclonal Antibody detects endogenous levels of ChAT protein.

Bioworld Molecular Weight: ~82kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (Rat brain)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ChAT) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (Mouse brain)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ChAT) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C

Alternative Name: Choline O acetyltransferase; Choline O acetyltransferase; Acetyl CoA choline O acetyltransferase; Acetyl CoA:choline O acetyltransferase; ChAT; CHOACTase; Choline acetylase; choline acetyltransferase; CMS1A; CMS1A2; EC 2.3.1.6; OTTHUMP00000019583; OTTHUMP00000019584; CLAT_HUMAN.

Immunogen: KLH conjugated synthetic peptide derived from human ChAT:451-550/748

Modification: Unmodified

Conjugate: Unconjugated