Product Description

CHCHD3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS71298-50, BS71298-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NX63

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:200

Background: The protein encoded by this gene is an inner mitochondrial membrane scaffold protein. Absence of the encoded protein affects the structural integrity of mitochondrial cristae and leads to reductions in ATP production, cell growth, and oxygen consumption. This protein is part of the mitochondrial contact site and cristae organizing system (MICOS). Several transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 30kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using CHCHD3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 40s., Confocal immunofluorescence analysis of U2OS cells using CHCHD3 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 600ug extracts of Mouse liver cells using 3ug CHCHD3 antibody. Western blot was performed from the immunoprecipitate using CHCHD3 antibody at a dilution of 1:1000.

Alternative Name: CHCHD3; MINOS3; Mic19; PPP1R22

Immunogen: Recombinant fusion protein of human CHCHD3(NP_060282.1).

Conjugate: Unconjugated

Modification: Unmodified