Product Description

CHEK2 (CHK2) Mouse Monoclonal Antibody

Sizes: 25μl, 100μl

Catalogue Numbers: MB4136-25, MB4136-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regμlators. The protein encoded by this gene is a cell cycle checkpoint regμlator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Category: Primary Antibody

Reactivity: Human, Dog, Mouse, Rat

Host: Mouse

Applications: FC, IF, IHC, IP, WB

Alternate Names: CDS1, CHK2, hCds1, HuCds1, LFS2, PP1425, RAD53

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Fμll length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell.

Dilution: WB 1:500~1000, IHC 1:150, IF 1:50~100, FLOW 1:100, IP 2ug/500μl

Purification: The antibody was affinity-purified from mouse ascites fluids or tissue cμlture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: IgG1

Molecular Weight: 60.7 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O96017

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only