Product Description

CHIP Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67252-50, MB67252-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The carboxy terminus of Hsc70-interacting protein (CHIP, STUB1) is a co-chaperone protein and functional E3 ubiquitin ligase that links the polypeptide binding activity of Hsp70 to the ubiquitin proteasome system. Cytoplasmic CHIP protein contains three 34-amino acid TPR (tetratricopeptide repeat) domains at its amino terminus and a carboxy-terminal U-box domain. CHIP interacts with the molecμlar chaperones Hsc70-Hsp70 and Hsp90 through its TPR domain, while E3 ubiquitin ligase activity is confined to the U-box domain. The binding of CHIP to Hsp70 can stall the folding of Hsp70 client proteins and concomitantly facilitate the U-box dependent ubiquitination of Hsp70-bound substrates. CHIP appears to play a central role in cell stress protection and is responsible for the degradation of disease-related proteins that include cystic fibrosis transmembrane conductance regμlator, p53, huntingtin and Ataxin-3, Tau protein, and α-synuclein.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Mouse

Applications: WB

Alternate Names: CHIP, E3 ubiquitin-protein ligase CHIP, Antigen NY-CO-7, CLL-associated antigen KW-8, Carboxy terminus of Hsp70-interacting protein, STIP1 homology and U box-containing protein 1

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human CHIP. The exact sequence is proprietary.

Dilution: WB (1/1000 - 1/2000)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of CHIP protein.

Molecular Weight: ~ 32 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q9UNE7

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only