Product Description

Chk1 (phospho-S317) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4040-50, BS4040-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Cell cycle events are regμlated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regμlators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regμlatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee 1 in vitro, providing evidence that the hyperphosphorylated form of Wee 1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1. Chk1 is phosphorylated on Serine 345 (S345) in response to UV, IR and hydroxyurea (HU). Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development and tumor suppression.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 317.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Chk1 (S317) polyclonal antibody detects endogenous levels of Chk1 protein when phosphorylated at Ser317.

Molecular Weight: ~ 56 kDa

Pathway: Cell Cycle Control G1 S Checkpoint, Cell Cycle G2 M DNA Damage Signaling Pathway, Epigenetic Writers and Erasers of Histone H3,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O14757

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only