Product Description

CHK2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90299-50, BS90299-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O96017(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC/IF, IHC, IP,

All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CHK2 polyclonal antibody detects endogenous levels of CHK2 protein.

Bioworld Molecular Weight: 62 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Chk2 on Hela cells lysates using anti-Chk2 antibody at 1/1,000 dilution., ICC staining Chk2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: Hucds1, hCds1, CHEK2, CDS 1, Cds1, Cds1 homolog, Checkpoint kinase 2, Checkpoint like protein CHK2, CHEK 2, Chek2, Chk 2, CHK2 checkpoint homolog (S. pombe), CHK2 checkpoint homolog, CHK2, hCds1, HuCds 1, LFS 2, LFS2, PP1425, RAD 53, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2, Serine/threonine-protein kinase Chk2,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified