Product Description

CHP2 Polyclonal Antibody

Catalogue Numbers: BS5661-50, BS5661-100

Sizes: 50µl, 100µl

Alternative Name: Calcineurin B homologous protein 2; Hepatocellular carcinoma-associated antigen 520; HCA520

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O43745

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: CHP2 (Calcineurin B homologous protein 2), also known as Hepatocellular carcinoma-associated antigen 520, is a 196 amino acid protein that contains four EF-hand domains and plays a potential role in transmembrane Na+/H+ exchange. By binding to and activating NHE-1, CHP2 increases the pH and works to protect cells from serum deprivation-induced death. Though not typically detected in normal tissues, CHP2 is highly expressed in malignantly transformed cells and is therefore considered to be a tumor-associated antigen. Ectopic expression of CHP2 promotes proliferation of HEK293 cells and knockdown of CHP2 mRNA in HepG2 cells inhibits cell proliferation. Like calcineurin B, CHP2 can bind to and stimulate phosphatase activity of calcineurin A and activate the calcineurin/NFAT signaling pathway.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CHP2 polyclonal antibody detects endogenous levels of CHP2 protein.

Bioworld Molecular Weight: ~ 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CHP2 polyclonal antibody at 1:500 dilution
Lane 1: HepG2 cell lysate
Lane 2: Mouse liver tissue lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 146-191 of Human CHP2.

Conjugate: Unconjugated

Modification: Unmodified