Product Description

CHRAC1 Polyclonal Antibody

Catalogue Numbers: BS61067-50, BS61067-100

Sizes: 50µl, 100µl

Alternative Name: Chromatin accessibility complex protein 1; CHRAC-1; Chromatin accessibility complex 15 kDa protein; CHRAC-15; HuCHRAC15; DNA polymerase epsilon subunit p15; CHRAC1; CHRAC15

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NRG0

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators. CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex),which increases the accessibility of nucleosomal DNA in an ATP-dependent manner. Unlike other known chromatin remodelling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing. This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array. In the presence of CHRAC, the nucleosomal ATPase ISWI catalyses several ATP-dependent transitions of chromatin structure.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CHRAC1 polyclonal antibody detects endogenous levels of CHRAC1 protein.

Bioworld Molecular Weight: ~ 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CHRAC1 polyclonal antibody at 1:500 dilution Lane 1: HEK293T whole cell lysate
Lane 2: RAW264.7 whole cell lysate Lane 3: PC12 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human CHRAC1.

Conjugate: Unconjugated

Modification: Unmodified