CHRNG Polyclonal Antibody-BS71453

CHRNG Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS71453-50, BS71453-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P07510

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: The mammalian muscle-type acetylcholine receptor is a transmembrane pentameric glycoprotein with two alpha subunits, one beta, one delta, and one epsilon (in adult skeletal muscle) or gamma (in fetal and denervated muscle) subunit. This gene, which encodes the gamma subunit, is expressed prior to the thirty-third week of gestation in humans. The gamma subunit of the acetylcholine receptor plays a role in neuromuscular organogenesis and ligand binding and disruption of gamma subunit expression prevents the correct localization of the receptor in cell membranes. Mutations in this gene cause Escobar syndrome and a lethal form of multiple pterygium syndrome. Muscle-type acetylcholine receptor is the major antigen in the autoimmune disease myasthenia gravis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using CHRNG antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10001 dilution.
Lysates/proteins: 26ug per lane.
Blocking buffer: 4% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.

Bioworld Molecular Weight: 60KDa

Specificity: Polyclonal Antibodies

Alternative Name: CHRNG; ACHRG

Immunogen: Recombinant fusion protein of human CHRNG(NP_005190.4).

Conjugate: Unconjugated

Modification: Unmodified