Product Description

CHST6 Polyclonal Antibody

Catalogue Numbers: BS61148-50, BS61148-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9GZX3

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. These enzymes differ in their tissue distributions and substrate specificities, although the gene structure (number and length of exons) is similar among family members. Carbohydrate sulfotransferase 5 (CHST5) (also referred to as GlcNAc6ST-3 or IGn6ST) and carbohydrate sulfotransferase 6 (CHST6) (also referred to as GlcNAc6ST-5 or Cgn6ST) are predominantly expressed in the intestine and cornea, respectively. They are highly homologous and both are orthologs of the murine CHST5. CHST5 and CHST6 may be the result of gene duplication. They catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. CHST5 preferably mediates the sulfation of short carbohydrates and O-linked sugars of mucin-type acceptors. CHST6 mediates the sulfation of keratan in the cornea, which is important in maintaining corneal transparency.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CHST6 polyclonal antibody detects endogenous levels of CHST6 protein.

Extra Notes: Western blot (WB) analysis of CHST6 pAb at 1:500 dilution
Lane1:U-87MG whole cell lysate(40ug)
Lane2:The spinal cord tissue lysate of Rat(40ug)

Bioworld Molecular Weight: ~ 52kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Carbohydrate sulfotransferase 6 (EC:2.8.2.-); Corneal N-acetylglucosamine-6-O-sulfotransferase; C-GlcNAc6ST; hCGn6ST; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4-beta; GST4-beta; N-acetylglucosamine 6-O-sulfotransferase 5; GlcNAc6ST-5; Gn6st-5; CHST6

Immunogen: A synthetic peptide corresponding to residues in Human CHST6.

Conjugate: Unconjugated

Modification: Unmodified