Product Description

Chymotrypsin Activity Colorimetric Assay Kit

Sizes: 48T, 96T

Catalogue Numbers: E-BC-K845-M-48, E-BC-K845-M-96

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Category: Cell Metabolism Assays

Storage and Stability: This product can be stored at -20°C for 12 months with shading light.

Research Areas: Amino Acids and Proteins

Result Type: Enzyme activity

Detection Range: 0.028-1.01 U/L

Detection Instrument: Microplate reader

Detection Type: Colorimetric method

Tested Sample Types: Tissue

Assay Length: 1 h 10 min

Sensitivity: 0.028 U/L

Test Principle: Chymotrypsin is a serine trypsin secreted by the pancreas, which is mainly used for the decomposition of proteins during digestion. It specifically hydrolyzes peptide bonds of aromatic amino acids (such as phenylalanine, tryptophan, and tyrosine) in proteins to generate smaller peptides or amino acids for further absorption by the body. In addition, chymotrypsin is widely used in protein analysis and research in the laboratory, especially for protein sequencing and structural analysis.
The detection principle of this kit is: The substrate was converted to a colored substance after chymotrypsin catalysis, and the OD value was detected at a wavelength of 405 nm, and the enzyme activity was calculated from the OD value of the standard.

Inter CV: 1.2-9.7%

Intra CV: 1.8-4.0%

Research Use Only