Product Description

CIB1 Polyclonal Antibody

Catalogue Numbers: BS60061-50, BS60061-100

Sizes: 50µl, 100µl

Alternative Name: Calcium and integrin-binding protein 1; Calcium- and integrin-binding protein; CIBP; Calmyrin; DNA-PKcs-interacting protein; Kinase-interacting protein; KIP; SNK-interacting protein 2-28; SIP2-28; CIB1; CIB; KIP; PRKDCIP

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99828

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Platelets regulate the function of Integrin α2b/β3 (GPIIb/IIIa), the platelet Fibrinogen receptor, which is involved in the binding of proteins to integrin cytoplasmic domains. A novel protein, CIB, for calcium- and integrin-binding protein (also designated as Kip for kinase interacting protein, SIP2-28 and DNA-PKcs interacting protein), binds specifically at the cytoplasmic domain of α2b by a number of positively charged residues in its binding site. Binding of CIB to the α2b is affected by fluctuations in the intracellular calcium concentration. In aggregated platelets, endogenous CIB and α2b/β3 translocate to the Triton X-100-insoluble cytoskeleton, demonstrating that the cellular localization of CIB is regulated. CIB also binds to DNA-PKcs, which is a nu-clear protein serine/threonine kinase that plays a role in the DNA repair and recombination process of lymphoid development. Fnk also binds to the CIB, suggesting that CIB may be a regulatory subunit of polo-like kinases. CIB shows significant homology to calcineurin B and calmodulin, and its mRNA levels are ubiquitously expressed in various human tissues.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: CIB1 polyclonal antibody detects endogenous levels of CIB1 protein.

Bioworld Molecular Weight: ~ 22 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CIB1 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: H9C2 whole cell lysate
Lane 3: Rat brain tissue lysate

Immunogen: A synthetic peptide corresponding to residues in Human CIB1.

Conjugate: Unconjugated

Modification: Unmodified