CISD1 Polyclonal Antibody-BS71677

CISD1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS71677-50, BS71677-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NZ45

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:1000 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The encoded protein has been localized to the outer membrane of mitochondria and is thought to play a role in regulation of oxidation. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 16KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of various lysates, using CISD1 antibody at 1:1420 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunofluorescence analysis of NIH/3T3 cells using CISD1 Rabbit pAb at dilution of 1:150. Blue: DAPI for nuclear staining., Immunofluorescence analysis of PC-12 cells using CISD1 Rabbit pAb at dilution of 1:150. Blue: DAPI for nuclear staining.

Alternative Name: CISD1; C10orf70; MDS029; ZCD1; mitoNEET

Immunogen: Recombinant fusion protein of human CISD1(NP_060934.1).

Conjugate: Unconjugated

Modification: Unmodified