CISH Polyclonal Antibody-BS65401

CISH Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS65401-50, BS65401-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9NSE2

Host: Rabbit

Reactivity: Rat, Mouse

Applications: WB, IHC

Background: The suppressor of cytokine signaling (SOCS) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway. The SOCS family consists of at least 8 members including the originally identified cytokine-inducible SH2-containing protein (CIS1), as well as SOCS1-7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation, and immune responses. CISH/CIS1, the first described member of the SOCS family, is induced by a number of cytokines including IL-2, IL-3, GM-CSF, and EPO. The CISH protein appears as a doublet around 32 and 37 kDa, the nature of which is unknown. CISH binds to phosphorylated cytokine receptors and can inhibit Stat5 activity. Expression of CISH is regulated by Stat5, thereby providing feedback modulation. Transgenic mice overexpressing CISH display phenotypes similar to Stat5 knockouts, including defects in mammary gland development and in T and NK cell regulation. Research studies have shown that polymorphisms within the CISH gene are associated with susceptibility to infectious diseases.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: CISH polyclonal antibody detects endogenous levels of CISH protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CISH polyclonal antibody at 1:500 dilution
Lane 1: The Liver tissue lysate of Mouse (40ug)
Lane 2: The Kidney tissue lysate of Rat (40ug)
Lane 3: The Liver tissue lysate of Rat (40ug), Immunohistochemistry of paraffin-embedded Human Liver cancer using CISH antibody at dilution of 1:50.

Bioworld Molecular Weight: ~ 32 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Cytokine-inducible SH2-containing protein; CIS; CIS-1; Protein G18; Suppressor of cytokine signaling; SOCS; CISH; G18

Immunogen: Synthetic peptide, corresponding to Human CISH.

Conjugate: Unconjugated

Modification: Unmodified