CK5+CK6 Polyclonal Antibody-BS65864

CK5+CK6 Polyclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65864-50, BS65864-100

Swiss-Prot: P02538

Host: Rabbit

CK5+CK6 Polyclonal Antibody-BS65864

Storage and Stability: Store at 4°C short term. Aliquot and store at -37°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: Keratins 5, 6 are members of type II keratins family that are specifically expressed in the inner root sheath of hair follicles. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Keratin 18 is memeber of the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family.

Purification and Purity: affinity purified by Protein A

Specificity: CK5+CK6 Polyclonal Antibody detects endogenous levels of CK5+CK6 protein.

Bioworld Molecular Weight: ~62kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (Mouse liver)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK5+CK6) Polyclonal Antibody, Unconjugated at 1:400, Paraformaldehyde-fixed, paraffin embedded (mouse brain)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK5+CK6) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C

Alternative Name: CK5+6; Cytokeratin 5 + 6; Cytokeratin 5; Keratin, type II cytoskeletal 5; CK-5; Keratin-5; K5; 58 kDa cytokeratin; KRT5; CK 5; DDD; EBS 2; EBS2; K5; Keratin 5 (epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types); Keratin 5; Keratin Type II Cytoskeletal 5; Keratin5; KRT 5; KRT 5A; KRT5.KRT6A; CK6A; CK6B; CK6C; CK6D; CK 6E; K6A; K6C; K6D; KRT6C; Cytokeratin 6a; Cytokeratin 6B; Cytokeratin 6C; Cytokeratin 6D; Cytokeratin 6E; Cytokeratin6; k6a keratin; K6B; K6b keratin; K6c keratin; K6d keratin; K6e keratin; k6hf; Keratin 6 isoform K6e; Keratin 6C; Keratin 6E; Keratin K6h; Keratin type II cytoskeletal 6A; Keratin type II cytoskeletal 6B; Keratin type II cytoskeletal 6C; Keratin type II cytoskeletal 6D; Keratin type II cytoskeletal 6E; KRT6; KRT6A; KRT6B; KRT6C; KRT6D; KRT6E; MGC102925.

Immunogen: KLH conjugated synthetic peptide derived from human CK5+CK6:161-220/590

Modification: Unmodified

Conjugate: Unconjugated