Product Description

CLASP1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90307-50, BS90307-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q7Z460 Human;Q80t V8 Mouse;Entrez Gene: 304740 Rat

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.

Purification and Purity: Protein A purified.

Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Specificity: CLASP1 polyclonal antibody detects endogenous levels of CLASP1 protein.

Bioworld Molecular Weight: 169 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of CLASP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive:
Lane 1: Daudi
Lane 2: HepG2, ICC staining CLASP1 in SH-SY-5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with YTHDF1 polyclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc and trade; 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Alternative Name: hOrbit1, CLASP1, KIAA0622, MAST1, 1700030C23Rik, 5730583A19Rik, B130045P17Rik, CLAP1, clasp1, CLIP associating protein 1, CLIP associating protein CLASP1, CLIP-associating protein 1, Cytoplasmic linker associated protein 1, Cytoplasmic linker-associated protein 1, DKFZp686D1968, DKFZp686H2039, FLJ33821, FLJ41222, hOrbit1, KIAA0622, MAST1, MGC131895, mKIAA0622, Multiple asters 1, Multiple asters homolog 1, Protein Orbit homolog 1 antibody

Immunogen: Recombinant protein within human CLASP1 aa 1-200.

Conjugate: Unconjugated

Modification: Unmodified