Product Description

Claudin 1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16429-50, BZ16429-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: O95832

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 23 kDa; Observed MW: 23 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Claudin 1 in Hela lysates using Claudin 1 antibody., Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using Claudin 1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right., Western blot analysis of Claudin 1 in HeLa lysates using Claudin 1 antibody. The Lane on the right is blocked with the synthesized peptide., Immunohistochemistry analysis of paraffin-embedded Mouse Colon tissue, using Claudin 1 antibody. High-pressure and temperature Tris-EDTA pH 9.0 was used for antigen retrieval.

Alternative Name: CLDN1; CLD1; SEMP1; Claudin-1; Senescence-associated epithelial membrane protein

Immunogen: The antiserum was produced against synthesized peptide derived from human Claudin 1. AA range:162-211

Conjugate: Unconjugated

Modification: Unmodified