Claudin 4 Monoclonal Antibody-MB66158

Claudin 4 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66158-50, MB66158-100

Product: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Swiss-Prot: O14493

Host: Mouse

Reactivity: Human

Applications: WB, IHC

All Applications: WB (1/500 - 1/1000), IHC (1/100 - 1/300)

Background: Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact made up of network of strands that act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Claudin 4 protein.

Bioworld Molecular Weight: ~ 23 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Claudin 4 expression in LNCaP (A) whole cell lysates., Immunohistochemical analysis of Claudin 4 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of Claudin 4 staining in human pancreatic carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Alternative Name: CPER; CPETR1; WBSCR8; Claudin-4; Clostridium perfringens enterotoxin receptor; CPE-R; CPE-receptor; Williams-Beuren syndrome chromosomal region 8 protein

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Claudin 4. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified