Product Description

Claudin-5 (S201) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1069-50, BS1069-100

Sizes: 50µl, 100µl

Swiss-Prot: O00501

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

Background: The Claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-5 is expressed in the endothelial junctions of the rat liver and in junctions of acinar cells of the pancreas. Human claudin-5 is abundantly expressed in adult lung, heart and skeletal muscle and is deleted in patients with velocardiofacial syndrone, which is characterized by cleft palate, facial dysmorphology and conotruncal heart defects.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Claudin 5 (S201) polyclonal antibody detects endogenous levels of Claudin 5 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Claudin-5 (S201) polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate treated with LPS
Lane 2: Mouse liver tissue lysate
Lane 3: Rat lung tissue lysate, Immunohistochemistry (IHC) analyzes of Claudin-5 (S201) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 23 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Claudin5; Claudin 5; Transmembrane protein deleted in VCFS; TMDVCF; CLDN5; AWAL; TMVCF

Immunogen: Synthetic peptide, corresponding to amino acids 171-220 of Human Claudin-5.

Conjugate: Unconjugated

Modification: Unmodified