Product Description

Cleaved-Caspase 3 p17 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16405-50, BZ16405-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P42574

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC-F, IHC-P, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 32 kDa; Observed MW: 17 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cleaved-Caspase3 in various lysates using Cleaved-Caspase3 p17 antibody., Immunohistochemistry analysis of paraffin-embedded mouse colon tissue using Cleaved-Caspase3 p17 antibody High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Alternative Name: CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 3. AA range:126-175

Conjugate: Unconjugated

Modification: Cleaved