Product Description

Cleaved-Notch 1 (V1754) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1050-50, ES1050-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: notch 1(NOTCH1) Homo sapiens This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymph

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF, IHC, ELISA

Alternate Names: NOTCH1; TAN1; Neurogenic locus notch homolog protein 1; Notch 1; hN1; Translocation-associated notch protein TAN-1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Notch 1. AA range:1735-1784

Isotype: IgG

Target: Notch-1,

Specificity: Cleaved-Notch 1 (V1754) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 1 protein resulting from cleavage adjacent to V1754.

Dilution: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.

Observed Band (KD): 110kD

Fields: Endocrine resistance, Notch signaling pathway, Th1 and Th2 cell differentiation, Thyroid hormone signaling pathway, Prion disease, Human papillomavirus infection, Pathways in cancer, MicroRNAs in cancer, Breast cancer

Function: Disease: Defects in NOTCH1 are a cause of aortic valve disease [MIM:109730]. The disorder consists of an early developmental defect in the aortic valve and a later de-repression of calcium deposition that causes progressive aortic valve disease. Calcification of the aortic valve is the third leading cause of heart disease in adults. The incidence increases with age, and it is often associated with a bicuspid aortic valve present in 1-2% of the population. Disease: NOTCH1 truncation is associated with T-cell acute lymphoblastic leukemia. Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiat

Subcellular Location: Cell membrane, Single-pass type I membrane protein, [Notch 1 intracellular domain]: Nucleus Following proteolytical processing NICD is translocated to the nucleus. Nuclear location may require MEGF10.

Storage and Stability: -20°C/1 year

Gene Name: NOTCH1

Protein Name: Neurogenic locus notch homolog protein 1

Human Gene ID: 4851

Human SwissProt Number: P46531

Mouse Gene ID: 18128

Mouse SwissProt Number: Q01705

Rat SwissProt Number: Q07008

Research Use Only