Product Description

Cleaved-NOTCH1 (V1754) Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-30054-60, E-AB-30054-120, E-AB-30054-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: Cleaved-NOTCH1 (V1754)

Target Synonym: hN1; Neurogenic locus Notch homolog protein 1; NICD; NOTC1; Notch 1; Notch 1 intracellular domain; Notch homolog 1 translocation associated (Drosophila); notch1; TAN1; Translocation-associated notch protein TAN-1

Research Areas: Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Developmental Biology, Neuroscience, Stem Cells

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human, Mouse, Rat

Application: WB, IHC-p, IF, ELISA

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P46531

Background: This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development.

Concentration: 1mg/mL

Immunogen: Synthesized peptide derived from the Internal region of human Notch 1

Buffer: PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

Purification Method: Affinity purification

Dilution: WB 1:500-2000, IHC 1:50-300, IF 1:50-300

Calculated MW: 273kDa

Observed MW: 110kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only