Product Description

Cleaved PARP Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90309-50, BS90309-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P09874(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC IP, FC

All Applications: WB:1:1,000-1:2,000
FC:1:50-1:100
ICC:1:50-1:200

Background: Poly(ADP-ribose) polymerase-1 (PARP-1), also designated PARP, is a nuclear DNA-binding zinc finger protein that influences DNA repair, DNA replication, modulation of chromatin structure, and apoptosis. In response to genotoxic stress, PARP-1 catalyzes the transfer of ADP-ribose units from NAD(+) to a number of acceptor molecules including chromatin. PARP-1 recognizes DNA strand interruptions and can complex with RNA and negatively regulate transcription. Actinomycin D- and etoposide-dependent induction of caspases mediates cleavage of PARP-1 into a p89 fragment that traverses into the cytoplasm. Apoptosis-inducing factor (AIF) translocation from the mitochondria to the nucleus is PARP-1-dependent and is necessary for PARP-1-dependent cell death. PARP-1 deficiencies lead to chromosomal instability due to higher frequencies of chromosome fusions and aneuploidy, suggesting that poly(ADP-ribosyl)ation contributes to the efficient maintenance of genome integrity. This antibody recognizes the apoptosis-specific 89 kDa catalytic domain fragment, but it does not recognize the full-length PARP-1 or the 24 kDa DNA binding domain fragment.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Cleaved PARP polyclonal antibody detects endogenous levels of Cleaved PARP protein.

Bioworld Molecular Weight: 89 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cleaved PARP on diferent lysates using anti-Cleaved PARP antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: A549, Flow cytometric analysis of Hela cells with Cleaved PARP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Alternative Name: PARP-1, ARTD1, ADPRT 1, PARP1, ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT 1, ADPRT, ADPRT1, APOPAIN, ARTD1, NAD(+) ADP-ribosyltransferase 1, PARP, PARP-1, PARP1, PARP1, Poly [ADP-ribose] polymerase 1, Poly ADP ribose polymerase 1, Poly[ADP-ribose] synthase 1, PPOL, SCA1,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Cleaved