CLIA Kit for Adrenocorticotropic Hormone (ACTH)
Sizes: 24T, 48T, 96T
Catalogue Numbers: CCA836Ra-24, CCA836Ra-48, CCA836Ra-96
Citations, Manuals and MSDS Available upon request.
Featured Series: Competitive kit
Target: Adrenocorticotropic Hormone
Alternative Names: Corticotropin; Adrenocorticotropin
Target Species: Rat
Uniprot: P01194
Featured Series Function: Detects small molecule
Specificity: Reactive with Rat ACTH / Adrenocorticotropic Hormone
Method: Chemiluminescence
Detection principle: Competitive Inhibition
Detection
range: 11.72-3000pg/mL
Sensitivity: 4.52pg/mL
Assay Time: 2h
Sample Size: 50uL
Recommended/Predicted
Sample Types: Serum, Plasma and other Biological Fluids
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Adrenocorticotropic Hormone (ACTH).
No significant cross-reactivity or interference between Adrenocorticotropic Hormone (ACTH) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenocorticotropic Hormone (ACTH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Target: 1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.
Test principle: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Adrenocorticotropic Hormone (ACTH). A competitive inhibition reaction is launched between biotin labeled Adrenocorticotropic Hormone (ACTH) and unlabeled Adrenocorticotropic Hormone (ACTH) (Standards or samples) with the pre-coated antibody specific to Adrenocorticotropic Hormone (ACTH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenocorticotropic Hormone (ACTH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Adrenocorticotropic Hormone (ACTH) level in the sample or standard.
Research Area: Endocrinology; Hormone metabolism;
Research Use Only