CLIA Kit for Ferroportin (FPN)
Size: 96T
Catalogue Number: USCC489Mu-96
Citations, Manuals and MSDS Available upon request.
Target: Ferroportin (FPN)
Alternative Names: SLC40A1; FPN1; HFE4; IREG1; MTP1; SLC11A3; Solute Carrier Family 40,Member 1; Iron-Regulated Transporter
Target Species: Mouse
Detection range: 0.156-10ng/mL
Sensitivity: 0.057ng/mL
Uniprot: Q9JHI9
Gene ID: 53945
Featured Series Function: Detects protein (regular version)
Recommended/Predicted Sample Types: Tissue Homogenates, Cell Lysates and other Biological Fluids
Assay Time: 2h, 40min
Method: Chemiluminescence
Specificity: Reactive with Mouse FPN / Ferroportin
Detection principle: Double-antibody Sandwich
Sample Size: 100uL
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Ferroportin (FPN).
No significant cross-reactivity or interference between Ferroportin (FPN) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferroportin (FPN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferroportin (FPN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
Test principle: The microplate provided in this kit has been pre-coated with an antibody specific to Ferroportin (FPN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ferroportin (FPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ferroportin (FPN) level in the sample or standard.;
Research Area: Metabolic pathway; Infection immunity; Hematology;
Research Use Only