CLIA Kit for Neutrophil gelatinase-associated lipocalin (NGAL)
Sizes: 24T, 48T, 96T
Catalogue Numbers: SCB388Mu-24, SCB388Mu-48, SCB388Mu-96
Citations, Manuals and MSDS Available upon request.
Featured Series: Sandwich kit
Target: Neutrophil gelatinase-associated lipocalin
Alternative Names: Lipocalin-2; LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2
Target Species: Mouse
Uniprot: P11672
Gene ID: 16819
Featured Series Function: Detects protein (regular version)
Specificity: Reactive with Mouse NGAL / Neutrophil gelatinase-associated lipocalin
Method: Chemiluminescence
Detection principle: Double-antibody Sandwich
Detection
range: 27.4-20,000pg/mL
Sensitivity: 10.2pg/mL
Assay Time: 2h, 40min
Sample Size: 100uL
Recommended/Predicted
Sample Types: Serum, Plasma, Urine, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Neutrophil gelatinase-associated lipocalin (NGAL).
No significant cross-reactivity or interference between Neutrophil gelatinase-associated lipocalin (NGAL) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neutrophil gelatinase-associated lipocalin (NGAL) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neutrophil gelatinase-associated lipocalin (NGAL) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Target: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
Test principle: The microplate provided in this kit has been pre-coated with an antibody specific to Neutrophil gelatinase-associated lipocalin (NGAL). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Neutrophil gelatinase-associated lipocalin (NGAL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Neutrophil gelatinase-associated lipocalin (NGAL) level in the sample or standard.;
Research Area: Infection immunity;
Research Use Only