CLIA Kit for Platelet Derived Growth Factor C (PDGFC)
Size: 96T
Catalogue Number: USCC920Hu-96
Citations, Manuals and MSDS Available upon request.
Target: Platelet Derived Growth Factor C (PDGFC)
Alternative Names: PDGF-C; SCDGF; VEGF-E; Fallotein; Spinal cord-derived growth factor; Platelet-derived growth factor C, latent form; Platelet-derived growth factor C, receptor-binding form
Target Species: Human
Detection range: 1.37-1,000pg/mL
Sensitivity: 0.56pg/mL
Uniprot: Q9NRA1
Gene ID: 56034
Featured Series Function: Detects protein (regular version)
Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids
Assay Time: 2h, 40min
Method: Chemiluminescence
Specificity: Reactive with Human PDGFC / Platelet Derived Growth Factor C
Detection principle: Double-antibody Sandwich
Sample Size: 100uL
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor C (PDGFC).
No significant cross-reactivity or interference between Platelet Derived Growth Factor C (PDGFC) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor C (PDGFC) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor C (PDGFC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
Test principle: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Platelet Derived Growth Factor C (PDGFC). A competitive inhibition reaction is launched between biotin labeled Platelet Derived Growth Factor C (PDGFC) and unlabeled Platelet Derived Growth Factor C (PDGFC) (Standards or samples) with the pre-coated antibody specific to Platelet Derived Growth Factor C (PDGFC). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Platelet Derived Growth Factor C (PDGFC) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Platelet Derived Growth Factor C (PDGFC) level in the sample or standard.
Research Area: Cytokine;
Research Use Only