CLIA Kit for Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)
Sizes: 24T, 48T, 96T
Catalogue Numbers: SCM427Hu-24, SCM427Hu-48, SCM427Hu-96
Citations, Manuals and MSDS Available upon request.
Featured Series: Sandwich kit
Target: Ras Related C3 Botulinum Toxin Substrate 1
Alternative Names: TC-25; p21-Rac1; MIG5; Cell migration-inducing gene 5 protein; Ras-like protein TC25; p21-Rac1
Target Species: Human
Uniprot: P63000
Gene ID: 5879
Featured Series Function: Detects protein (regular version)
Specificity: Reactive with Human Rac1 / Ras Related C3 Botulinum Toxin Substrate 1
Method: Chemiluminescence
Detection principle: Double-antibody Sandwich
Detection
range: 13.7-10,000pg/mL
Sensitivity: 5.1pg/mL
Assay Time: 2h, 40min
Sample Size: 100uL
Recommended/Predicted
Sample Types: Tissue Homogenates, Cell Lysates and other Biological Fluids
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Ras Related C3 Botulinum Toxin Substrate 1 (Rac1).
No significant cross-reactivity or interference between Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Target: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.
Test principle: The microplate provided in this kit has been pre-coated with an antibody specific to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) level in the sample or standard.;
Research Area: Tumor immunity;
Research Use Only