Product Description

CLN6 (N258) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3102-50, BS3102-100

Sizes: 50µl, 100µl

Swiss-Prot: Q9NWW5

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: CLN6, a 311-amino acid protein, has 7 predicted transmembrane domains and is conserved across vertebrates. The CLN6 protein localizes to the endoplasmic reticulum but contributes to lysosomal function. Mutations in the CLN6 gene cause variant late-onset infantile neuronal ceroid lipofuscinosis (vLINCL), a lysosomal storage disorder marked by progressive mental deterioration and blindness; part of a group of severe inherited neurodegenerative disorders affecting children wherein lysosomes accumulate storage material, causing the death of neurons. CLN6 is one of eight proteins, including CLN1-8, that are associated with NCL.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: CLN6 (N258) polyclonal antibody detects endogenous levels of CLN6 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of CLN6 (N258) pAb at 1:500 dilution
Lane 1: SGC7901 whole cell lysate (40ug)
Lane 2: SK-OVCAR3 whole cell lysate (40ug)
Lane 3: MCF-7 whole cell lysate (40ug)
Lane 4: PC12 whole cell lysate (40ug)
Lane 5: CT26 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of CLN6 (N258) pAb in paraffin-embedded human stomach carcinoma tissue at 1:50.showing nuclear and strong cytoplasmic staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 40 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Ceroid-lipofuscinosis neuronal protein 6; Protein CLN6; CLN6

Immunogen: Synthetic peptide, corresponding to amino acids 229-274 of Human CLN6.

Conjugate: Unconjugated

Modification: Unmodified